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CD16 Polyclonal antibody

CD16 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16559-1-AP

Synonyms

CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2

Validation Data Gallery

  • WB analysis of human placenta using 16559-1-AP

    WB analysis of human placenta using 16559-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16559-1-AP (CD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16559-1-AP

    IHC staining of human liver using 16559-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16559-1-AP (CD16 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 16559-1-AP

    IHC staining of human liver using 16559-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16559-1-AP (CD16 Antibody) at dilution of 1:200 (under 40x lens).

  • FC experiment of U-937 using 16559-1-AP

    FC experiment of U-937 using 16559-1-AP

    1X10^6 U-937 cells were stained with 0.2ug CD16 antibody (16559-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16559-1-AP targets CD16 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD16 fusion protein Ag9787
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC017865
Gene symbol FCGR3A
Gene ID (NCBI) 2214
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.

Protocols

Product Specific Protocols
WB protocol for CD16 antibody 16559-1-APDownload protocol
IHC protocol for CD16 antibody 16559-1-APDownload protocol
FC protocol for CD16 antibody 16559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Hum Reprod

Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.

Authors - Yanling Gou
View Article
mouseIHC

Food Funct

Amelioration of clinical course and demyelination in the cuprizone mouse model in relation to ketogenic diet.

Authors - Ning Zhang
View Article