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CD16 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 16559-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

CD16 Antibody WB human placenta tissue 16559-1-AP

human placenta tissue were subjected to SDS PAGE followed by western blot with 16559-1-AP( CD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

CD16 Antibody IHC human liver tissue 16559-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16559-1-AP( CD16 Antibody) at dilution of 1:200 (under 10x lens).

CD16 Antibody IHC human liver tissue 16559-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16559-1-AP( CD16 Antibody) at dilution of 1:200 (under 40x lens).

CD16 Antibody FC U-937 cells 16559-1-AP

1X10^6 U-937 cells were stained with 0.2ug CD16 antibody (16559-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human
Positive WB detected in:
human placenta tissue
Positive IHC detected in:
human liver tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
U-937 cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD16 fusion protein Ag9787
Full name:
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight:
254aa,29 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
BC017865
Gene ID (NCBI):
2214
Gene symbol
FCGR3A
Synonyms
CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2

Background

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.


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