|Positive WB detected in||human placenta tissue|
|Positive IHC detected in||human liver tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||U-937 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16559-1-AP targets CD16 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD16 fusion protein Ag9787|
|Full Name||Fc fragment of IgG, low affinity IIIa, receptor (CD16a)|
|Calculated molecular weight||254 aa, 29 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC017865|
|Gene ID (NCBI)||2214|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.
Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.
Amelioration of clinical course and demyelination in the cuprizone mouse model in relation to ketogenic diet.
Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring.