CD16 Polyclonal antibody
CD16 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 16559-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human placenta tissue |
Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | U-937 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
16559-1-AP targets CD16 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD16 fusion protein Ag9787 |
Full Name | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
Calculated molecular weight | 254 aa, 29 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC017865 |
Gene symbol | FCGR3A |
Gene ID (NCBI) | 2214 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD16 antibody 16559-1-AP | Download protocol |
IHC protocol for CD16 antibody 16559-1-AP | Download protocol |
FC protocol for CD16 antibody 16559-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hum Reprod Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis. | ||
Food Funct Amelioration of clinical course and demyelination in the cuprizone mouse model in relation to ketogenic diet. | ||
Theranostics Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring. |