Anti-Human CD16 (3G8)

CD16 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1, k

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

3G8

Cat no : 65090-1-Ig

Synonyms

CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2



Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65090-1-Ig targets CD16 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1, k
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC017865
Gene symbol FCGR3A
Gene ID (NCBI) 2214
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.

Protocols

Product Specific Protocols
FC protocol for CD16 antibody 65090-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols