CD16 Recombinant monoclonal antibody

CD16 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83110-2-RR

Clone No.230337A4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CD16A, CD16a antigen, CD16-II, Fc gamma RIII, Fc gamma RIII alpha

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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-/ -

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Filter: AllWBIHC

WB analysis of human placenta using 83110-2-RR
human placenta tissue were subjected to SDS PAGE followed by western blot with 83110-2-RR (CD16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 83110-2-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83110-2-RR (CD16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 83110-2-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83110-2-RR (CD16 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 83110-2-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83110-2-RR (CD16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive WB detected in	human placenta tissue, Recombinant protein
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83110-2-RR targets CD16 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg31662 Product name: Recombinant Human FCGR3A/CD16a (F176V) protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 17-208 aa of BC017865 Sequence: GMRTEDLPKAVVFLEPQWYRVLEKDSVTLKCQGAYSPEDNSTQWFHNESLISSQASSYFIDAATVDDSGEYRCQTNLSTLSDPVQLEVHIGWLLLQAPRWVFKEEDPIHLRCHSWKNTALHKVTYLQNGKGRKYFHHNSDFYIPKATLKDSGSYFCRGLVGSKNVSSETVNITITQGLAVSTISSFFPPGYQ Predict reactive species
Full Name	Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated Molecular Weight	254 aa, 29 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC017865
Gene Symbol	CD16a
Gene ID (NCBI)	2214
ENSEMBL Gene ID	ENSG00000203747
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08637
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD16 is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.