Tested Applications
| Positive WB detected in | mouse liver tissue, mouse lung tissue, rat spleen tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue, rat small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83285-4-RR targets CD163 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0794 Product name: Recombinant Mouse CD163 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 39-1045 aa of NM_053094.2 Sequence: VTNAPGEMKKELRLAGGENNCSGRVELKIHDKWGTVCSNGWSMNEVSVVCQQLGCPTSIKALGWANSSAGSGYIWMDKVSCTGNESALWDCKHDGWGKHNCTHEKDAGVTCSDGSNLEMRLVNSAGHRCLGRVEIKFQGKWGTVCDDNFSKDHASVICKQLGCGSAISFSGSAKLGAGSGPIWLDDLACNGNESALWDCKHRGWGKHNCDHAEDVGVICLEGADLSLRLVDGVSRCSGRLEVRFQGEWGTVCDDNWDLRDASVVCKQLGCPTAISAIGRVNASEGSGQIWLDNISCEGHEATLWECKHQEWGKHYCHHREDAGVTCSDGADLELRLVGGGSRCAGIVEVEIQKLTGKMCSRGWTLADADVVCRQLGCGSALQTQAKIYSKTGATNTWLFPGSCNGNETTFWQCKNWQWGGLSCDNFEEAKVTCSGHREPRLVGGEIPCSGRVEVKHGDVWGSVCDFDLSLEAASVVCRELQCGTVVSILGGAHFGEGSGQIWGEEFQCSGDESHLSLCSVAPPLDRTCTHSRDVSVVCSRYIDIRLAGGESSCEGRVELKTLGAWGPLCSSHWDMEDAHVLCQQLKCGVAQSIPEGAHFGKGAGQVWSHMFHCTGTEEHIGDCLMTALGAPTCSEGQVASVICSGNQSQTLLPCSSLSPVQTTSSTIPKESEVPCIASGQLRLVGGGGRCAGRVEVYHEGSWGTVCDDNWDMTDANVVCKQLDCGVAINATGSAYFGEGAGAIWLDEVICTGKESHIWQCHSHGWGRHNCRHKEDAGVICSEFMSLRLTNEAHKENCTGRLEVFYNGTWGSIGSSNMSPTTVGVVCRQLGCADNGTVKPIPSDKTPSRPMWVDRVQCPKGVDTLWQCPSSPWKQRQASPSSQESWIICDNKIRLQEGHTDCSGRVEIWHKGSWGTVCDDSWDLNDAKVVCKQLGCGQAVKALKEAAFGPGTGPIWLNEIKCRGNESSLWDCPAKPWSHSDCGHKEDASIQCLPKMTSESHHGTGHPT Predict reactive species |
| Full Name | CD163 antigen |
| Calculated Molecular Weight | 121 kDa |
| Observed Molecular Weight | 160-170 kDa |
| GenBank Accession Number | NM_053094.2 |
| Gene Symbol | Cd163 |
| Gene ID (NCBI) | 93671 |
| RRID | AB_3670952 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q2VLH6-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD163 antibody 83285-4-RR | Download protocol |
| IHC protocol for CD163 antibody 83285-4-RR | Download protocol |
| WB protocol for CD163 antibody 83285-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CD163 Recombinant monoclonal antibody (240233A3), catalog number 83285-4-RR, has 10 total citations. These appear in journals including J Nanobiotechnology, Materials Today Bio, Acta Biomaterialia, International Journal of Oncology, Frontiers in Immunology, Journal of Nutritional Biochemistry, Journal of Cardiovascular Translational Research, Biochemical and Biophysical Research Communications, and Inflammation Research. The research fields span osteogenesis, nerve regeneration, hepatic ischemia-reperfusion injury, tumor immunology, cardiac fibrosis, macrophage polarization, diabetic wound healing, and osteoarthritis.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology The bone marrow mesenchymal stem cells derived migrasomes induced by Titania nanotubes surface serve as chemotaxis effect for osteogenesis. | ||
Mater Today Bio Fuel-maintenance coupling reprograms mitochondrial homeostasis to enable peripheral nerve regeneration. | ||
Mater Today Bio Pressure-sensitive composite bandages with high toughness, self-healability and strong tissues adhesion for rapid bone fixation and accelerated osteogenesis via immunomodulation and neovascularization. | ||
Acta Biomater M2 macrophage-derived exosome nanoplatform for targeted H(2)S delivery to alleviate hepatic ischemia-reperfusion injury via synergistic anti-oxidative, anti-inflammatory, and anti-ferroptotic effects. | ||
Int J Oncol Hypoxia‑induced exosomal CAMTA1 promotes radio‑resistance in MDA‑MB‑231 cells by regulating NRG1 to mediate M2 macrophage polarization. | ||
Front Immunol Zuojin capsule improves T cell exhaustion and tumor immune microenvironment of hepatocellular carcinoma through the mTOR-eIF4E/p70S6K-CDK1 pathway. |
Reviews
What customers say
Both reviewers report positive results. One user (5/5) used the antibody for immunofluorescence on FFPE mouse spleen tissue at a 1:200 dilution overnight at 4°C, paired with goat anti-rabbit secondary at 1:1000, and described it as working perfectly with clear images at 10x and 20x magnification. Another user (4/5) confirmed the antibody performed well in ELISA at a 1:20000 dilution. Overall, users find this CD163 antibody reliable across different applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
clotilde (Verified Customer) (07-22-2026) | This CD163 works perfectly in IF-P. The IF was performed on FFPE mouse spleen tissue slide with Dako antigen retrival pH9. Primary incubation was performed at 1:200 overnight (16h) at 4°C. Secondary incubation was performed with Goat anti-Rabbit at 1:1000 for 1h at RT. Image were taken with a Leica THUNDER at 10x and 20x magnification.
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Chinar (Verified Customer) (10-23-2025) | antibody worked well in ELISA
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