Home
Products
Primary Antibodies
Flow Cytometry Antibodies
Anti-Human CD177 Rabbit Recombinant Antibody, PBS Only (98086-1-PBS)

Anti-Human CD177 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human CD177 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98086-1-PBS-1MG

CD177 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98086-1-PBS

Clone No. 241339F4

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

NB1, Human neutrophil alloantigen 2a, HNA-2a, HNA2A, HNA 2a

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllFCAffinity

FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.
FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.
FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)
1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98086-1-RR
Biolayer interferometry (BLl) kinetic assays of 98086-1-RR against Human CD177 were performed. The affinity constant is 1.70 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CD177 in ?

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

98086-1-PBS targets CD177 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Recombinant Protein Predict reactive species
Full Name	CD177 molecule
Calculated Molecular Weight	437 aa, 46 kDa
GenBank Accession Number	BC029167
Gene Symbol	CD177
Gene ID (NCBI)	57126
RRID	AB_3672233
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8N6Q3
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD177, also known as NB1, HNA-2a or PRV-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the uPAR/CD59/Ly6 snake toxin superfamily (PMID: 17898576). It is expressed exclusively by neutrophils, neutrophilic metamyelocytes, and myelocytes (PMID: 17580308). CD177 interacts with β2 integrin CD11b/CD18 and CD11a/CD18, PECAM-1, and participates in neutrophil activation and transmigration (PMID: 17580308; 21193407). CD177 has been identified as a useful biomarker for myeloproliferative diseases (PMID: 17898576).