Validation Data Gallery
|Positive WB detected in||U-937 cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||RAW 264.7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 10554-1-AP is CD18 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD18 fusion protein Ag0861|
|Full Name||integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||85-90 kDa|
|GenBank accession number||BC005861|
|Gene ID (NCBI)||3689|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).
|Product Specific Protocols|
|WB protocol for CD18 antibody 10554-1-AP||Download protocol|
|IHC protocol for CD18 antibody 10554-1-AP||Download protocol|
|IF protocol for CD18 antibody 10554-1-AP||Download protocol|
|FC protocol for CD18 antibody 10554-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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