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- KD/KO Validated
CD18 Polyclonal antibody
CD18 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10554-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-937 cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue |
Positive FC detected in | RAW 264.7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 9 publications below |
IHC | See 8 publications below |
IF | See 7 publications below |
FC | See 2 publications below |
Product Information
The immunogen of 10554-1-AP is CD18 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD18 fusion protein Ag0861 |
Full Name | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
Calculated molecular weight | 85 kDa |
Observed molecular weight | 85-90 kDa |
GenBank accession number | BC005861 |
Gene symbol | ITGB2 |
Gene ID (NCBI) | 3689 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).
Protocols
Product Specific Protocols | |
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WB protocol for CD18 antibody 10554-1-AP | Download protocol |
IHC protocol for CD18 antibody 10554-1-AP | Download protocol |
IF protocol for CD18 antibody 10554-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Appl Mater Interfaces Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer. | ||
Theranostics ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.
| ||
Cardiovasc Res Myocardin-related transcription factor A (MRTF-A) regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice. | ||
Int J Mol Sci Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury. | ||
J Immunol PALLD Regulates Phagocytosis by Enabling Timely Actin Polymerization and Depolymerization. |