Tested Applications
| Positive WB detected in | U-937 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10554-1-AP targets CD18 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0861 Product name: Recombinant human CD18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 469-769 aa of BC005861 Sequence: ICRCDTGYIGKNCECQTQGRSSQELEGSCRKDNNSIICSGLGDCVCGQCLCHTSDVPGKLIYGQYCECDTINCERYNGQVCGGPGRGLCFCGKCRCHPGFEGSACQCERTTEGCLNPRRVECSGRGRCRCNVCECHSGYQLPLCQECPGCPSPCGKYISCAECLKFEKGPFGKNCSAACPGLQLSNNPVKGRTCKERDSEGCWVAYTLEQQDGMDRYLIYVDESRECVAGPNIAAIVGGTVAGIVLIGILLLVIWKALIHLSDLREYRRFEKEKLKSQWNNDNPLFKSATTTVMNPKFAES Predict reactive species |
| Full Name | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 85-90 kDa |
| GenBank Accession Number | BC005861 |
| Gene Symbol | CD18 |
| Gene ID (NCBI) | 3689 |
| ENSEMBL Gene ID | ENSG00000160255 |
| RRID | AB_2877736 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05107 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD18 antibody 10554-1-AP | Download protocol |
| IHC protocol for CD18 antibody 10554-1-AP | Download protocol |
| WB protocol for CD18 antibody 10554-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD18 Polyclonal antibody (Cat# 10554-1-AP) has 53 citations published across 44 journals, including Theranostics, Advanced Science, Cardiovascular Research, PNAS, Journal of Experimental Medicine, Journal of Biological Chemistry, and Frontiers in Immunology. Research fields encompass immunology (integrin signaling, macrophage and neutrophil biology), cardiovascular disease (atherosclerosis, cardiac fibrosis), neuroinflammation (cerebral malaria, spinal cord injury), oncology (gastric, breast, lung, ovarian cancer), nanomedicine and biomaterials, and inflammatory disorders (acute lung injury, rheumatoid arthritis).
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Integration of bioinformatics analysis and experimental verification reveals the role of neutrophil extracellular traps in silica nanoparticles-induced lung injury. | ||
Theranostics Comprehensive exome profiling identifies ARHGEF12 mutation as a driver in gastric cancer with ovarian metastasis | ||
Theranostics ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.
| ||
Adv Sci (Weinh) Ovarian Matrisome Dynamics and αvβ3-Mediated Regulation in Early Follicular Development. | ||
Adv Sci (Weinh) Irisin-Encapsulated Mitochondria-Targeted Biomimetic Nanotherapeutics for Alleviating Acute Kidney Injury | ||
Research (Wash D C) Regulating Blood Clot Fibrin Films to Manipulate Biomaterial-Mediated Foreign Body Responses |
