CD18 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD18 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 10554-1-AP

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

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Validation Data Gallery

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  WB analysis using 10554-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10554-1-AP (CD18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10554-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP (CD18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10554-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP (CD18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 10554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 10554-1-AP (CD18 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 10554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 10554-1-AP (CD18 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 10554-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug CD18 antibody (10554-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: U-937 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue
• Positive FC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 8 publications below
• IF: See 7 publications below
• FC: See 2 publications below

Product Information

The immunogen of 10554-1-AP is CD18 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD18 fusion protein Ag0861
• Full Name: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• Calculated molecular weight: 85 kDa
• Observed molecular weight: 85-90 kDa
• GenBank accession number: BC005861
• Gene symbol: ITGB2
• Gene ID (NCBI): 3689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Publications

Species	Application	Title
human	FC	J Exp Med; Layilin augments integrin activation to promote antitumor immunity.; Authors - Kelly M Mahuron
human	WB,IF	ACS Appl Mater Interfaces; Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer.; Authors - Yali Jun
human	WB,IHC,IF	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang ; KD Validated
mouse	WB	Cardiovasc Res; Myocardin-related transcription factor A (MRTF-A) regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice.; Authors - Li Liu
human,mouse	WB, IHC	Int J Mol Sci; Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.; Authors - Vincent Yi-Fong Su
human	FC	J Immunol; PALLD Regulates Phagocytosis by Enabling Timely Actin Polymerization and Depolymerization.; Authors - Hai-Min Sun