CD18 Polyclonal antibody

CD18 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10554-1-AP

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7



Tested Applications

Positive WB detected inU-937 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 10554-1-AP is CD18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD18 fusion protein Ag0861
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Calculated molecular weight 85 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC005861
Gene symbol ITGB2
Gene ID (NCBI) 3689
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

Product Specific Protocols
WB protocol for CD18 antibody 10554-1-APDownload protocol
IHC protocol for CD18 antibody 10554-1-APDownload protocol
IF protocol for CD18 antibody 10554-1-APDownload protocol
FC protocol for CD18 antibody 10554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Biol Chem

Interaction between the integrin Mac-1 and signal regulatory protein α (SIRPα) mediates fusion in heterologous cells.

Authors - Nataly P Podolnikova
humanWB,IF

ACS Appl Mater Interfaces

Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer.

Authors - Yali Jun
human,mouseWB, IHC

Int J Mol Sci

Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.

Authors - Vincent Yi-Fong Su
IHC

Tissue Eng Part C Methods

A Practical Procedure for the In Vitro Generation of Human Osteoclasts and Their Characterization.

Authors - Johanna F A Husch
humanWB

Am J Cancer Res

Bufalin inhibits glycolysis-induced cell growth and proliferation through the suppression of Integrin β2/FAK signaling pathway in ovarian cancer.

Authors - Haoran Li
humanWB

J Biol Chem

The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites.

Authors - Fumitoshi Irie