CD18 Recombinant monoclonal antibody

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• CD18 Recombinant monoclonal antibody (87449-1-RR)

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  WB analysis using 87449-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87449-1-RR (ITB2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 87449-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87449-1-RR (CD18 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 87449-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87449-1-RR (CD18 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 87449-1-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells (postive) and HeLa cells (Negative) using CD18 antibody (87449-1-RR, Clone: 252742C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 87449-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87449-1-RR against Human CD18 were performed. The affinity constant is below 1 pM.

CD18 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 87449-1-RR

Clone No.252742C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ITGB2, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Integrin beta 2, Integrin beta-2, LAD

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Tested Applications

• Positive WB detected in: Jurkat cells, THP-1 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87449-1-RR targets CD18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10481

  Product name: Recombinant human ITB2 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011434 of

  Sequence: \

• Full Name: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• Observed Molecular Weight: 90-100 kDa
• Gene Symbol: CD18
• Gene ID (NCBI): 3689
• ENSEMBL Gene ID: ENSG00000160255
• RRID: AB_3745554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05107
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during the immune response, and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).