CD180 Monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP51097-1

  Sandwich ELISA standard curve of MP51097-1, Human CD180 Monoclonal Matched Antibody Pair - PBS only. 60768-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag28488. 60768-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP51097-1

  The mean CD180 concentration was determined to be 670.7 pg/mL in Ramos cell extract based on a 1.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP51097-1

  Cytometric bead array standard curve of MP51097-1, CD180 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60768-1-PBS. Detection antibody: 60768-2-PBS. Standard:Ag28488. Range: 0.391-100 ng/mL.

CD180 Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 60768-1-PBS

Clone No.3B1E8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Ly78, LY64, CD180 molecule, CD180 antigen, CD 180

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Product Information

60768-1-PBS targets CD180 as part of a matched antibody pair:

MP51097-1: 60768-1-PBS capture and 60768-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28488

  Product name: Recombinant human CD180 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 164-389 aa of BC109069

  Sequence: IKFPKDFPARNLKVLDFQNNAIHYISREDMRSLEQAINLSLNFNGNNVKGIELGAFDSTVFQSLNFGGTPNLSVIFNGLQNSTTQSLWLGTFEDIDDEDISSAMLKGLCEMSVESLNLQEHRFSDISSTTFQCFTQLQELDLTATHLKGLPSGMKGLNLLKKLVLSVNHFDQLCQISAANFPSLTHLYIRGNVKKLHLGVGCLEKLGNLQTLDLSHNDIEASDCCS

• Full Name: CD180 molecule
• Calculated Molecular Weight: 661 aa, 74 kDa
• GenBank Accession Number: BC109069
• Gene Symbol: CD180
• Gene ID (NCBI): 4064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99467
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.