CD19 Polyclonal antibody
CD19 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27949-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Daudi cells, Raji cells |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue, rat colon tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Raji cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 1 publications below |
Product Information
27949-1-AP targets CD19 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat samples.
Tested Reactivity | Human, Rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD19 fusion protein Ag27656 |
Full Name | CD19 molecule |
Calculated molecular weight | 556 aa, 61 kDa |
Observed molecular weight | 95 kDa |
GenBank accession number | BC006338 |
Gene symbol | CD19 |
Gene ID (NCBI) | 930 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD19 antibody 27949-1-AP | Download protocol |
IHC protocol for CD19 antibody 27949-1-AP | Download protocol |
IF protocol for CD19 antibody 27949-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Inflamm Regen Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling |