CD19 Polyclonal antibody

CD19 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27949-1-AP

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12



Tested Applications

Positive WB detected inDaudi cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue, rat colon tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

27949-1-AP targets CD19 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD19 fusion protein Ag27656
Full Name CD19 molecule
Calculated molecular weight 556 aa, 61 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC006338
Gene symbol CD19
Gene ID (NCBI) 930
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

Protocols

Product Specific Protocols
WB protocol for CD19 antibody 27949-1-APDownload protocol
IHC protocol for CD19 antibody 27949-1-APDownload protocol
IF protocol for CD19 antibody 27949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Inflamm Regen

Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling

Authors - Hirofumi Watanabe