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CD19 Polyclonal antibody

CD19 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, Rat, Mouse and More (3)





Cat no : 27949-1-AP


B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

Tested Applications

Positive WB detected inDaudi cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, mouse colon tissue, human lymphoma tissue, rat colon tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRaji cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27949-1-AP targets CD19 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD19 fusion protein Ag27656
Full Name CD19 molecule
Calculated Molecular Weight 556 aa, 61 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.


Product Specific Protocols
WB protocol for CD19 antibody 27949-1-APDownload protocol
IHC protocol for CD19 antibody 27949-1-APDownload protocol
IF protocol for CD19 antibody 27949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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