CD19 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD19 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27949-1-AP

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

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Validation Data Gallery

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  WB analysis of Raji using 27949-1-AP

  WB result of CD19 antibody (27949-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD19 transfected Raji cells.

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  WB analysis using 27949-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27949-1-AP (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27949-1-AP

  Raji cell (CD19 positive) and Jurkat (CD19 negative) were subjected to SDS PAGE followed by western blot with 27949-1-AP (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 27949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 27949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (27949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue, mouse colon tissue, human lymphoma tissue, rat colon tissue, rat thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 4 publications below

Product Information

27949-1-AP targets CD19 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD19 fusion protein Ag27656
• Full Name: CD19 molecule
• Calculated Molecular Weight: 556 aa, 61 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC006338
• Gene Symbol: CD19
• Gene ID (NCBI): 930
• RRID: AB_2881019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

Publications

Species	Application	Title
rat	IHC	J Adv Res; Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model; Authors - Haonan Ruan
human	IHC	Int J Mol Sci; CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing; Authors - Chen Su
human	WB	Int J Biol Macromol; Exosome co-delivery of a STING agonist augments immunogenicity elicited by CVB3 VP1 vaccine via promoting antigen cross-presentation of CD8 + DCs; Authors - Changwei Zhang
human	IHC	Inflamm Regen; Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling; Authors - Hirofumi Watanabe
mouse	IHC	Pharmaceuticals (Basel); Hydrogen Attenuates Inflammation by Inducing Early M2 Macrophage Polarization in Skin Wound Healing; Authors - Pengxiang Zhao