Anti-Mouse CD19 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of mouse splenocytes using 98287-1-RR (same clone as 98287-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD19 Rabbit RecAb (98287-1-RR, Clone:242301C6) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 98287-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98287-1-RR (same clone as 98287-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD19 Rabbit RecAb (98287-1-RR, Clone: 242301C6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98287-1-PBS in a different storage buffer formulation.

CD19 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98287-1-PBS

Clone No.242301C6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD19 antigen, 242301C6

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Product Information

98287-1-PBS targets CD19 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD19 antigen
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_009844.2
• Gene Symbol: Cd19
• Gene ID (NCBI): 12478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P25918
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).