CD1a Polyclonal antibody

CD1a Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17325-1-AP

Synonyms

CD1, CD1A, CD1a molecule, FCB6, HTA1, hTa1 thymocyte antigen, R4, T6

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Validation Data Gallery

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  IHC staining of human skin cancer using 17325-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17325-1-AP (CD1a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 17325-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17325-1-AP (CD1a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• IHC: See 2 publications below

Product Information

The immunogen of 17325-1-AP is CD1a Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD1a fusion protein Ag11304
• Full Name: CD1a molecule
• Calculated molecular weight: 327 aa, 37 kDa
• GenBank accession number: BC031645
• Gene symbol: CD1A
• Gene ID (NCBI): 909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1a is a 50-kDa glycoprotein. It has a heterodimer form with molecular weight of 65-70 kDa.

Publications

Species	Application	Title
human	IHC	Cancer Immunol Immunother; Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.; Authors - Pei Yang
human	IHC	Chin Med J (Engl); Hyperbaric oxygen treatment on keloid tumor immune gene expression.; Authors - Chun-Hu Wang

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