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CD1a Polyclonal antibody

CD1a Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17325-1-AP

Synonyms

CD1, CD1A, CD1a molecule, FCB6, HTA1, hTa1 thymocyte antigen, R4, T6

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CD1a Polyclonal antibody (17325-1-AP)

    IHC staining of human skin cancer using 17325-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17325-1-AP (CD1a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CD1a Polyclonal antibody (17325-1-AP)

    IHC staining of human skin cancer using 17325-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17325-1-AP (CD1a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17325-1-AP is CD1a Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD1a fusion protein Ag11304
Full Name CD1a molecule
Calculated molecular weight 327 aa, 37 kDa
GenBank accession numberBC031645
Gene symbol CD1A
Gene ID (NCBI) 909
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1a is a 50-kDa glycoprotein. It has a heterodimer form with molecular weight of 65-70 kDa.

Protocols

Product Specific Protocols
IHC protocol for CD1a antibody 17325-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article
humanIHC

Cancer Immunol Immunother

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Authors - Pei Yang
View Article
humanIHC

Chin Med J (Engl)

Hyperbaric oxygen treatment on keloid tumor immune gene expression.

Authors - Chun-Hu Wang
View Article
mouseIHC

Stem Cell Res Ther

Human umbilical cord mesenchymal stem cell treatment alleviates symptoms in an atopic dermatitis-like mouse model

Authors - Chunting Hua
View Article