CD1a Polyclonal antibody

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  IHC staining of human skin cancer using 17325-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17325-1-AP (CD1a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 17325-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17325-1-AP (CD1a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD1a Polyclonal Antibody for IHC, ELISA

Cat No. 17325-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Synonyms

CD1, CD1A, CD1a molecule, FCB6, HTA1, hTa1 thymocyte antigen, R4, T6

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Tested Applications

• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 5 publications below

Product Information

17325-1-AP targets CD1a in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD1a fusion protein Ag11304
• Full Name: CD1a molecule
• Calculated Molecular Weight: 327 aa, 37 kDa
• GenBank Accession Number: BC031645
• Gene Symbol: CD1A
• Gene ID (NCBI): 909
• RRID: AB_10638479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06126
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1a is a 50-kDa glycoprotein. It has a heterodimer form with molecular weight of 65-70 kDa.

Publications

Species	Application	Title
pig	WB	Emerg Microbes Infect; CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis; Authors - Xin Chen
human	IHC	Cancer Immunol Immunother; Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.; Authors - Pei Yang
human	IHC	Chin Med J (Engl); Hyperbaric oxygen treatment on keloid tumor immune gene expression.; Authors - Chun-Hu Wang
mouse	IHC	Stem Cell Res Ther; Human umbilical cord mesenchymal stem cell treatment alleviates symptoms in an atopic dermatitis-like mouse model; Authors - Chunting Hua
human	IHC	BMC Med Genomics; Establishment and validation of an immune infiltration predictive model for ovarian cancer; Authors - Zhenxia Song
human,mouse	IHC	Aging (Albany NY); Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma; Authors - Xiaonan Zhang