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CD1a Polyclonal antibody

CD1a Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17325-1-AP

Synonyms

CD1, CD1A, CD1a molecule, FCB6, HTA1, hTa1 thymocyte antigen, R4, T6

Validation Data Gallery

  • WB analysis of K-562 using 17325-1-AP

    WB analysis of K-562 using 17325-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17325-1-AP (CD1a antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 17325-1-AP

    IHC staining of human skin cancer using 17325-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17325-1-AP (CD1a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 17325-1-AP

    IHC staining of human skin cancer using 17325-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17325-1-AP (CD1a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17325-1-AP is CD1a Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD1a fusion protein Ag11304
Full Name CD1a molecule
Calculated molecular weight 327 aa, 37 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC031645
Gene symbol CD1A
Gene ID (NCBI) 909
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1a is a 50-kDa glycoprotein. It has a heterodimer form with molecular weight of 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for CD1a antibody 17325-1-APDownload protocol
IHC protocol for CD1a antibody 17325-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols