CoraLite®647 Anti-Human CD2 (TS1/8)

CD2 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

TS1/8

Cat no : CL647-65192

Synonyms

CD2, CD2 molecule, Erythrocyte receptor, FLJ46032, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11



Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65192-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65192 targets CD2 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
GenBank accession numberBC033583
Gene symbol CD2
Gene ID (NCBI) 914
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
FC protocol for CL647 CD2 antibody CL647-65192Download protocol
Standard Protocols
Click here to view our Standard Protocols