Tested Applications
| Positive FC detected in | human PBMCs | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension | 
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65192 targets CD2 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human | 
| Host / Isotype | Mouse / IgG1, kappa | 
| Class | Monoclonal | 
| Type | Antibody | 
| Immunogen | N/APredict reactive species | 
| Full Name | CD2 molecule | 
| Calculated Molecular Weight | 351 aa, 39 kDa | 
| GenBank Accession Number | BC033583 | 
| Gene Symbol | CD2 | 
| Gene ID (NCBI) | 914 | 
| ENSEMBL Gene ID | ENSG00000116824 | 
| RRID | AB_2935242 | 
| Conjugate | FITC Plus Fluorescent Dye | 
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm | 
| Form | Liquid | 
| Purification Method | Affinity purification | 
| UNIPROT ID | P06729 | 
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. | 
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. | 
Background Information
CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD2 antibody FITC-65192 | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 




