CD20 Recombinant monoclonal antibody

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• CD20 Recombinant monoclonal antibody (86480-1-RR)

CD20 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86480-1-RR

Clone No.251207B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35

• 

  WB analysis using 86480-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86480-1-RR (MS4A1,CD20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Jurkat using 86480-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji / Jurkat cells using CD20 antibody (86480-1-RR, Clone: 251207B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86480-1-RR targets CD20 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002807
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• RRID: AB_3744914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11836-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.