Anti-Human CD20 Rabbit Recombinant Antibody

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• Anti-Human CD20 Rabbit Recombinant Antibody (98771-1-RR)

CD20 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98771-1-RR

Clone No.253584A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35

• 

  FC experiment of human PBMCs using 98771-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Rabbit RecAb (98771-1-RR, Clone:253584A1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98771-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Rabbit RecAb (98771-1-RR, Clone:253584A1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98771-1-RR targets CD20 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• RRID: AB_3746337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11836
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).