CD200 Polyclonal antibody

CD200 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14057-1-AP

Synonyms

CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein

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Validation Data Gallery

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  WB analysis of HEK-293 using 14057-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14057-1-AP (CD200 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14057-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14057-1-AP (CD200 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14057-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14057-1-AP (CD200 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14057-1-AP targets CD200 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD200 fusion protein Ag5045
• Full Name: CD200 molecule
• Calculated molecular weight: 269 aa, 30 kDa
• Observed molecular weight: 41-47 kDa
• GenBank accession number: BC022522
• Gene symbol: CD200
• Gene ID (NCBI): 4345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Stem Cell Res Ther; Psoralen alleviates radiation-induced bone injury by rescuing skeletal stem cell stemness through AKT-mediated upregulation of GSK-3β and NRF2.; Authors - Bo-Feng Yin
mouse	IHC	Pharmaceutics; Minoxidil Nanoparticles Targeting Hair Follicles Enhance Hair Growth in C57BL/6 Mice.; Authors - Yoshihiro Oaku