FITC Anti-Human CD206 (15-2)
CD206 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human
Applications
FC
Conjugate
FITC Fluorescent Dye
CloneNo.
15-2
Cat no : FITC-65155
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human monocyte-derived dendritic cells cells |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 7 publications below |
Product Information
FITC-65155 targets CD206 in FC applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Purified human mannose receptor |
Full Name | mannose receptor, C type 1 |
Calculated Molecular Weight | 166 kDa |
GenBank Accession Number | NM_002438 |
Gene Symbol | CD206 |
Gene ID (NCBI) | 4360 |
RRID | AB_2920499 |
Conjugate | FITC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 494 nm / 520 nm |
Form | Liquid |
Purification Method | The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
Storage Buffer | PBS with 0.1% sodium azide. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.
Protocols
Product Specific Protocols | |
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FC protocol for FITC CD206 antibody FITC-65155 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochim Biophys Acta Mol Basis Dis Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages | ||
Front Immunol Chamaejasmenin B, an Inhibitor for Metastatic Outgrowth, Reversed M2-Dominant Macrophage Polarization in Breast Tumor Microenvironment. | ||
Pharm Biol Schizandrin A ameliorates cognitive functions via modulating microglial polarisation in Alzheimer's disease mice. | ||
Cancer Sci Homeobox A7 promotes esophageal squamous cell carcinoma progression through C-C motif chemokine ligand 2-mediated tumor-associated macrophage recruitment | ||
J Biochem Mol Toxicol KLF4-induced upregulation of SOCS1 ameliorates myocardial ischemia/reperfusion injury by attenuating AC16 cardiomyocyte damage and enhancing M2 macrophage polarization | ||