Anti-Human CD206 (15-2)

CD206 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

15-2

Cat no : 65155-1-Ig

Synonyms

CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1

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Validation Data Gallery

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  FC experiment of human monocyte-derived dendritic cells using 65155-1-Ig

  Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 0.5 ug Anti-Human CD206 (65155-1-Ig, Clone:15-2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or stained with 0.5 ug mouse IgG1 isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human monocyte-derived dendritic cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65155-1-Ig targets CD206 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified human mannose receptor
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 166 kDa
• GenBank Accession Number: NM_002438
• Gene Symbol: MRC1
• Gene ID (NCBI): 4360
• RRID: AB_2918437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.