Validation Data Gallery
|Positive WB detected in
|DC2.4 cells, Raji cells, Ramos cells, Daudi cells
|Positive IHC detected in
|human tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|human tonsillitis tissue
|Western Blot (WB)
|WB : 1:2000-1:8000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 60208-1-Ig is CD23 Fusion Protein expressed in E. coli.
|Host / Isotype
|Mouse / IgG1
|CD23 fusion protein Ag0425
|Fc fragment of IgE, low affinity II, receptor for (CD23)
|Calculated molecular weight
|321 aa, 36 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.
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