CD23 Monoclonal antibody, PBS Only

CD23 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C7C9

Cat no : 60208-2-PBS

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

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Validation Data Gallery

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  WB analysis of Raji using 60208-2-Ig (same clone as 60208-2-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60208-2-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-2-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-2-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60208-2-PBS targets CD23 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD23 fusion protein Ag0425
• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: FCER2
• Gene ID (NCBI): 2208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.