CD23 Recombinant monoclonal antibody

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  WB analysis using 84024-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84024-2-RR (CD23,FCER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 84024-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84024-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84024-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83996-4-RR against Human CD23 were performed. The affinity constant is 3.66 nM.

CD23 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84024-2-RR

Clone No.241269B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, Daudi cells, U-937 cells, NIH/3T3 cells, RAW 264.7 cells, mouse spleen tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84024-2-RR targets CD23 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: CD23
• Gene ID (NCBI): 2208
• RRID: AB_3671593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06734
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD23, also known as low-affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centers, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells