Tested Applications
| Positive WB detected in | mouse liver tissue, mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32150-1-AP targets CD24 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg2104 Product name: Recombinant Mouse CD24 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 27-53 aa of NM_009846.2 Sequence: NQTSVAPFPGNQNISASPNPSNATTRG Predict reactive species |
| Full Name | CD24a antigen |
| Calculated Molecular Weight | 8 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | NM_009846.2 |
| Gene Symbol | CD24 |
| Gene ID (NCBI) | 12484 |
| RRID | AB_3670209 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P24807 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD24 antibody 32150-1-AP | Download protocol |
| WB protocol for CD24 antibody 32150-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD24 Polyclonal antibody (Cat# 32150-1-AP) has 1 total citation. It appears in the International Journal of Biological Macromolecules (Impact Factor 8.7), published in February 2026. The cited study investigates how CD24 overexpression confers resistance to Pyrotinib through inhibiting autophagy in HER2-positive breast cancer patients, placing this citation within the research fields of oncology, drug resistance mechanisms, and autophagy regulation.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol CD24 overexpression confers resistance to Pyrotinib through inhibiting autophagy in patients with HER2-positive breast cancer. |
