FC experiment of mouse splenocytes using 65121-1-Ig
1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD24 (M1/69) (65121-1-Ig, Clone: M1/69) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD24 (M1/69) (65121-1-Ig, Clone: M1/69) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65121-1-Ig targets CD24 in FC applications and shows reactivity with mouse samples.
Tested Reactivity
mouse
Host / Isotype
Rat / IgG2b, kappa
Class
Monoclonal
Type
Antibody
Immunogen
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes Predict reactive species
FC experiment of mouse splenocytes using 65121-1-Ig
1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD24 (M1/69) (65121-1-Ig, Clone: M1/69) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD24 Monoclonal antibody
Catalog Number
65121-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
