|Positive WB detected in||NK-92 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Sample-dependent, check data in validation data gallery|
16677-1-AP targets CD244 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD244 fusion protein Ag9860|
|Full Name||CD244 molecule, natural killer cell receptor 2B4|
|Calculated molecular weight||365 aa, 41 kDa|
|Observed molecular weight||60-70 kDa|
|GenBank accession number||BC053985|
|Gene ID (NCBI)||51744|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).
A case report of clonal EBV-like memory CD4+ T cell activation in fatal checkpoint inhibitor-induced encephalitis.
Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.