Anti-Human CD244 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98133-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD244 Rabbit Recombinant Antibody (98133-1-RR, Clone: 241260G10) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98133-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD244 Rabbit Recombinant Antibody (98133-1-RR, Clone:241260G10) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98133-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98133-1-RR against Human CD244 were performed. The affinity constant is 1.29 nM.

CD244 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98133-1-RR

Clone No.241260G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98133-1-RR targets CD244 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD244 molecule, natural killer cell receptor 2B4
• Calculated Molecular Weight: 365 aa, 41 kDa
• GenBank Accession Number: BC053985
• Gene Symbol: CD244
• Gene ID (NCBI): 51744
• RRID: AB_3672279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9BZW8
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).