CD247 Polyclonal antibody

CD247 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 12837-2-AP

Synonyms

CD247, CD247 molecule, CD3 ZETA, CD3H, CD3Q, CD3Z, T cell receptor T3 zeta chain, T3Z, TCRZ

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Validation Data Gallery

• 

  WB analysis of Jurkat using 12837-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12837-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 12837-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12837-2-AP

  IP result of anti-CD247 (IP:12837-2-AP, 3ug; Detection:12837-2-AP 1:700) with Jurkat cells lysate 1200ug.

• 

  IHC staining of human tonsillitis using 12837-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12837-2-AP (CD247 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12837-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12837-2-AP (CD247 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 12837-2-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 12837-2-AP (CD247 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 12837-2-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD247 antibody (12837-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: Jurkat cells, human spleen tissue, mouse spleen tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Jurkat cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

12837-2-AP targets CD247 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD247 fusion protein Ag3889
• Full Name: CD247 molecule
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC025703
• Gene Symbol: CD247
• Gene ID (NCBI): 919
• RRID: AB_2244279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD247, also known as CD3Z (T-cell surface glycoprotein CD3 zeta chain), belongs to the CD3Z/FCER1G family. CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Publications

Species	Application	Title
human	WB	Front Immunol; The Transferrin Receptor-Directed CAR for the Therapy of Hematologic Malignancies.; Authors - Zilong Guo
mouse	WB	PLoS One; Liver Sinusoidal Endothelial Cell-Mediated CD8 T Cell Priming Depends on Co-Inhibitory Signal Integration over Time.; Authors - Julita Kaczmarek
human	IHC	Front Immunol; Distinct immune and inflammatory response patterns contribute to the identification of poor prognosis and advanced clinical characters in bladder cancer patients; Authors - Zhenglin Chang
human	FC	Cytotherapy; Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma; Authors - Wenjing Luo
human	WB,IP	Cell Rep; Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance; Authors - Xueliang Gao
mouse	WB	Nat Commun; Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function; Authors - Yuman Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Haizhen (Verified Customer) (10-15-2019)

specific for human CD3 zeta protein

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000 for western blot
• Cell Tissue Type: leukemia cell line

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