Validation Data Gallery
|Positive WB detected in||Jurkat cells, human spleen tissue, mouse spleen tissue|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Jurkat cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12837-2-AP targets CD247 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD247 fusion protein Ag3889|
|Full Name||CD247 molecule|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16 kDa|
|GenBank accession number||BC025703|
|Gene ID (NCBI)||919|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD247, also known as CD3Z (T-cell surface glycoprotein CD3 zeta chain), belongs to the CD3Z/FCER1G family. CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.
|Product Specific Protocols|
|WB protocol for CD247 antibody 12837-2-AP||Download protocol|
|IHC protocol for CD247 antibody 12837-2-AP||Download protocol|
|IF protocol for CD247 antibody 12837-2-AP||Download protocol|
|IP protocol for CD247 antibody 12837-2-AP||Download protocol|
|FC protocol for CD247 antibody 12837-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Haizhen (Verified Customer) (10-15-2019)
specific for human CD3 zeta protein