Anti-Human CD25/IL-2RA (BC96) Mouse IgG2a Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 65620-1-MR (same clone as 65620-1-PBS)

  1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD25 (BC96) Mouse IgG2a Recombinant Antibody (65620-1-MR, Clone: BC96) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD25 (BC96) Mouse IgG2a Recombinant Antibody (65620-1-MR, Clone: BC96) and PE-conjugated Goat Anti-Mouse IgG (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 65620-1-PBS in a different storage buffer formulation.

CD25/IL-2RA Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65620-1-PBS

Clone No.BC96

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

CD25, BC96, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha

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Product Information

65620-1-PBS targets CD25/IL-2RA in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: interleukin 2 receptor, alpha
• Calculated Molecular Weight: 272 aa, 31 kDa
• GenBank Accession Number: BC156627
• Gene Symbol: IL2RA
• Gene ID (NCBI): 3559
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).