Anti-Human CD27 (O323)

CD27 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1









Cat no : 65132-1-Ig


CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65132-1-Ig targets CD27 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD27 molecule
Calculated molecular weight 29 kDa
GenBank accession numberBC012160
Gene symbol CD27
Gene ID (NCBI) 939
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide, pH 7.2.
Storage ConditionsStore at 2-8°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.


Product Specific Protocols
FC protocol for CD27 antibody 65132-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols