CD27 Recombinant antibody, PBS Only

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  WB analysis using 85066-5-RR (same clone as 85066-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85066-5-RR (CD27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Ramos using 85066-5-RR (same clone as 85066-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

CD27 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IF-P, Indirect ELISA

Cat No. 85066-5-PBS

Clone No.242731F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IF-P, Indirect ELISA

Synonyms

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

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Product Information

85066-5-PBS targets CD27 in WB, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD27 molecule
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC012160
• Gene Symbol: CD27
• Gene ID (NCBI): 939
• ENSEMBL Gene ID: ENSG00000139193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26842
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.