Anti-Human CD27 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 98346-3-RR (same clone as 98346-3-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 Rabbit RecAb (98346-3-RR, Clone:242731G8) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98346-3-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98346-3-RR (same clone as 98346-3-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 Rabbit RecAb (98346-3-RR, Clone:242731G8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98346-3-PBS in a different storage buffer formulation.

CD27 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98346-3-PBS

Clone No.242731G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

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Product Information

98346-3-PBS targets CD27 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD27 molecule
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC012160
• Gene Symbol: CD27
• Gene ID (NCBI): 939
• ENSEMBL Gene ID: ENSG00000139193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26842
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.