APC Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2)

CD274 (PD-L1, B7-H1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

10F.9G2

Cat no : APC-65073

Synonyms

B7 H1, Cd274, CD274 antigen, PD L1, Pdcd1l1, Pdcd1lg1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using APC-65073

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20ug APC-Anti-Mouse CD274 (PD-L1, B7-H1) (APC-65073, clone 10F.9G2) (red) or 0.20 ug APC-Rat IgG2b isotype control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

APC-65073 targets CD274 (PD-L1, B7-H1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD274 antigen
• GenBank accession number: BC066841
• Gene symbol: Cd274
• Gene ID (NCBI): 60533
• Conjugate: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.