PE Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2)

CD274 (PD-L1, B7-H1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b


Mouse and More (2)




PE Fluorescent Dye



Cat no : PE-65073


B7 H1, Cd274, CD274 antigen, PD L1, Pdcd1l1, Pdcd1lg1

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 2 publications below

Product Information

PE-65073 targets CD274 (PD-L1, B7-H1) in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivityhuman, mouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD274 antigen
GenBank Accession NumberBC066841
Gene Symbol Cd274
Gene ID (NCBI) 60533
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.


Product Specific Protocols
FC protocol for PE CD274 (PD-L1, B7-H1) antibody PE-65073Download protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Pharmacol

Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT

Authors - Xing Chang


FXR agonist GW4064 enhances anti-PD-L1 immunotherapy in colorectal cancer

Authors - Lu Lu