Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2)

CD274 (PD-L1, B7-H1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

10F.9G2

Cat no : 65073-1-Ig

Synonyms

B7 H1, Cd274, CD274 (PD-L1, B7-H1), CD274 antigen, PD L1, Pdcd1l1, Pdcd1lg1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 65073-1-Ig

  1x10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD274 (PD-L1, B7-H1) (65073-1-Ig, Clone:10F.9G2) and FITC-anti Rat IgG2b at dilution 1:100 and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 stimulated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65073-1-Ig targets CD274 (PD-L1, B7-H1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD274 antigen
• GenBank Accession Number: BC066841
• Gene Symbol: Cd274
• Gene ID (NCBI): 60533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.