PE Anti-Mouse CD275 (B7-H2) (HK5.3)

CD275 (B7-H2) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

HK5.3

Cat no : PE-65114

Synonyms

AU044799, B7 H2, B7h, B7-H2, B7RP 1, BG071784, CD275, GI50, GL50, GL50 B, ICOS L, icos ligand, Icosl, Icoslg, KIAA0653, LICOS, Ly115l, mKIAA0653

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using PE-65114

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.06 ug PE Anti-Mouse CD275 (B7-H2) (PE-65114, Clone: HK5.3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65114

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.06 ug PE-Rat IgG2a isotype control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65114 targets CD275 (B7-H2) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse B7-RP1 transfected cell line
• Full Name: icos ligand
• GenBank Accession Number: BC029227
• Gene Symbol: Icosl
• Gene ID (NCBI): 50723
• RRID: AB_2883903
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD275, also known as B7-H2, ICOSLG or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. CD275 is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of CD275/ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).