B7-H3/CD276 Polyclonal antibody

• Featured Product
• KD/KO Validated

B7-H3/CD276 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14453-1-AP

Synonyms

4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 14453-1-AP

  WB result of B7-H3 antibody (14453-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B7-H3 transfected HEK-293 cells.

• 

  WB analysis using 14453-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14453-1-AP (B7-H3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14453-1-AP (B7-H3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14453-1-AP (B7-H3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14453-1-AP (B7-H3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14453-1-AP (B7-H3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14453-1-AP (B7-H3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14453-1-AP (B7-H3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human medulloblastoma using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 14453-1-AP (B7-H3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human medulloblastoma using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 14453-1-AP (B7-H3 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, U2OS cells
• Positive IHC detected in: human prostate cancer tissue, human testis tissue, human medulloblastoma tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

14453-1-AP targets B7-H3/CD276 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B7-H3/CD276 fusion protein Ag6006
• Full Name: CD276 molecule
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC062581
• Gene Symbol: CD276
• Gene ID (NCBI): 80381
• RRID: AB_2073577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Publications

Species	Application	Title
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	WB	J Cell Physiol; Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis.; Authors - Lipeng Tang
human	IHC	CNS Neurosci Ther; PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.; Authors - Hao Zhang
human	IHC	J Cancer; The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway.; Authors - Zhangao Li
human	IHC	Med Oncol; Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.; Authors - Miao Li
human	WB	J Oncol; Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.; Authors - Qiu-Ting Li