Tested Applications
| Positive WB detected in | HEK-293 cells, LNCaP cells, U2OS cells |
| Positive IHC detected in | human prostate cancer tissue, human medulloblastoma tissue, human testis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14453-1-AP targets B7-H3/CD276 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6006 Product name: Recombinant human CD276 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-181 aa of BC062581 Sequence: LVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDG Predict reactive species |
| Full Name | CD276 molecule |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC062581 |
| Gene Symbol | CD276 |
| Gene ID (NCBI) | 80381 |
| RRID | AB_2073577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5ZPR3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for B7-H3/CD276 antibody 14453-1-AP | Download protocol |
| WB protocol for B7-H3/CD276 antibody 14453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-B7-H3 antibody (Cat# 14453-1-AP) has 24 citations across journals including J Extracell Vesicles, Acta Pharm Sin B, J Transl Med, Front Immunol, iScience, Commun Biol, Sci Rep, and J Cancer. Research fields span oncology (breast, gastric, colorectal, glioma, lung, and pancreatic cancers), immune checkpoint therapy, macrophage biology, skin photodamage, psoriasis, and reproductive biology.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Engineered CAR-T-Derived Exosomes Co-Delivering miR-145 and Cytotoxic Proteins for Targeted Solid Tumour Therapy. | ||
Acta Pharm Sin B PKCα inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability | ||
J Transl Med B7-H3 interaction with cMet enhances lipolysis to drive malignant progression of glioma via pSTAT3-ATGL axis. | ||
Chin Med J (Engl) B7-H3 confers stemness characteristics to gastric cancer cells by promoting glutathione metabolism through AKT/pAKT/Nrf2 pathway
| ||
Front Immunol Neoadjuvant immunochemotherapy improves clinical outcomes of patients with esophageal cancer by mediating anti-tumor immunity of CD8+ T (Tc1) and CD16+ NK cells | ||
CNS Neurosci Ther PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma. |
