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B7-H3 Polyclonal antibody

B7-H3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14453-1-AP

Synonyms

4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352



Tested Applications

Positive WB detected inLNCaP cells, PC-3 cells, HEK-293 cells, U2OS cells
Positive IHC detected inhuman tonsillitis tissue, human medulloblastoma tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

14453-1-AP targets B7-H3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B7-H3 fusion protein Ag6006
Full Name CD276 molecule
Calculated molecular weight 57 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC062581
Gene symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Protocols

Product Specific Protocols
WB protocol for B7-H3 antibody 14453-1-APDownload protocol
IHC protocol for B7-H3 antibody 14453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

BMC Urol

Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse.

Authors - Kai Li
humanWB

J Oncol

Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.

Authors - Qiu-Ting Li
humanIHC

Med Oncol

Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.

Authors - Miao Li
humanIHC

J Cancer

The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway.

Authors - Zhangao Li
humanWB

J Cell Physiol

Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis.

Authors - Lipeng Tang