|Positive WB detected in||LNCaP cells, PC-3 cells, HEK-293 cells, U2OS cells|
|Positive IHC detected in||human tonsillitis tissue, human medulloblastoma tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
14453-1-AP targets B7-H3 in WB, IHC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||B7-H3 fusion protein Ag6006|
|Full Name||CD276 molecule|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC062581|
|Gene ID (NCBI)||80381|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse.
Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.
Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.
The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway.
J Cell Physiol
Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis.