Anti-Human CD279 (PD-1) (J110)

CD279 (PD-1) Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

J110

Cat no : 65119-1-Ig

Synonyms



Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65119-1-Ig targets CD279 (PD-1) in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name programmed cell death 1
Calculated molecular weight 288 aa, 32 kDa
GenBank accession numberBC074740
Gene symbol PDCD1
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

Protocols

Product Specific Protocols
FC protocol for CD279 (PD-1) antibody 65119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols