FcZero-rAb™ Biotin Anti-Human CD28 (CD28.2) Rabbit IgG Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-FcA65621

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 (UCHT1), and 0.25 ug Biotin Anti-Human CD28 (CD28.2) Rabbit IgG RecAb (Biotin-FcA65621, Clone: CD28.2) or Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD28 FcZero-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-FcA65621

Clone No.CD28.2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD28 molecule, T-cell-specific surface glycoprotein CD28, Tp44

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA65621 targets CD28 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD28 molecule
• Calculated Molecular Weight: 220 aa, 25 kDa
• GenBank Accession Number: BC093698
• Gene Symbol: CD28
• Gene ID (NCBI): 940
• ENSEMBL Gene ID: ENSG00000178562
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).