Anti-Human CD29 (TS2/16)

CD29 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (2)

Applications

FC

Conjugate

Unconjugated

Publications

2

CloneNo.

TS2/16

Cat no : 65191-1-Ig

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

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Validation Data Gallery

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  FC experiment of human PBMCs using 65191-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD29 (65191-1-Ig, Clone:TS2/16) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (red), or unstained (blue). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65191-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD29 (65191-1-Ig, Clone:TS2/16) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (right), or unstained (left). Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

65191-1-Ig targets CD29 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: BC020057
• Gene Symbol: ITGB1
• Gene ID (NCBI): 3688
• RRID: AB_2918461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Publications

Species	Application	Title
human	FC	Exp Ther Med; Adipose-derived mesenchymal stem cell exosomes inhibit transforming growth factor-β1-induced collagen synthesis in oral mucosal fibroblasts.; Authors - Junjie Liu
mouse	FC	Dis Markers; Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis; Authors - Baihe Han