CoraLite® Plus 488 Anti-Human CD29 (TS2/16)

CD29 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

2

CloneNo.

TS2/16

Cat no : CL488-65191

Synonyms

ITGB1, ITB1, Integrin beta-1, Integrin beta 1, GPIIA

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using CL488-65191

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD29 (CL488-65191, Clone:TS2/16) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124, Clone: MOPC-21). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65191

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD29 (CL488-65191, Clone:TS2/16) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124, Clone: MOPC-21). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes and monocytes were gated.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

CL488-65191 targets CD29 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: BC020057
• Gene Symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• RRID: AB_2883214
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Publications

Species	Application	Title
		Mol Med Rep; Ghrelin promotes cardiomyocyte differentiation of adipose tissue‑derived mesenchymal stem cells by DDX17‑mediated regulation of the SFRP4/Wnt/β‑catenin axis; Authors - Gui-Bo Liu
human	FC	J Bioenerg Biomembr; LNX2 involves in the role of ghrelin to promote the neuronal differentiation of adipose tissue-derived mesenchymal stem cells; Authors - Gui-Bo Liu