CD2AP Polyclonal antibody

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  WB analysis using 32089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32089-1-AP (CD2AP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 32089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32089-1-AP (CD2AP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 32089-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cd2ap antibody (32089-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CD2AP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 32089-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

CMS, CD2-associated protein, CD2 Associated Protein, Cas ligand with multiple SH3 domains, Adapter protein CMS

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Tested Applications

• Positive WB detected in: A431 cells, mouse testis tissue, Daudi cells, Jurkat cells, K-562 cells, rat testis tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32089-1-AP targets CD2AP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36919

  Product name: Recombinant human CD2AP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 400-639 aa of NM_012120.2

  Sequence: NLLRSSGTVYPKRPEKPVPPPPPIAKINGEVSSISSKFETEPVSKLKLDSEQLPLRPKSVDFDSLTVRTSKETDVVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTHSPEKILKLPKEEDSANLKPSELKKDTCYSPKPSVYLSTPSSASKANTTAFLTPLEIKAKVETDDVKKNSLDELRAQIIELLCIVEALKKDHGKELEKLRKDLEEEKTMRSNLEMEIEKLKKAVLSS

• Full Name: CD2-associated protein
• Calculated Molecular Weight: 71 kDa，639aa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: NM_012120.2
• Gene Symbol: CD2AP
• Gene ID (NCBI): 23607
• RRID: AB_3670188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Y5K6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).