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Mouse CD2AP Recombinant monoclonal antibody, PBS Only (86727-1-PBS)

Mouse CD2AP Recombinant monoclonal antibody, PBS Only

Name: Mouse CD2AP Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 86727-1-PBS-100UG

CD2AP Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 86727-1-PBS

Clone No.251428G2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CD2 associated protein, CD2-associated protein, Mesenchyme-to-epithelium transition protein with SH3 domains 1, METS 1, Mets1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Proteintech Guarantee

Filter: AllWBIHCIF-PAffinity

WB analysis using 86727-1-RR (same clone as 86727-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86727-1-RR (Cd2ap antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 86727-1-RR (same clone as 86727-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86727-1-RR (CD2AP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 86727-1-RR (same clone as 86727-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86727-1-RR (CD2AP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 86727-1-RR (same clone as 86727-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86727-1-RR (CD2AP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 86727-1-RR (same clone as 86727-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86727-1-RR (CD2AP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse kidney using 86727-1-RR (same clone as 86727-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CD2AP antibody (86727-1-RR, Clone: 251428G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CD2AP antibody (86727-1-RR, Clone: 251428G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
IF Staining of mouse kidney using 86727-1-RR (same clone as 86727-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CD2AP antibody (86727-1-RR, Clone: 251428G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CD2AP antibody (86727-1-RR, Clone: 251428G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86727-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86727-1-RR
Biolayer interferometry (BLl) kinetic assays of 86727-1-RR against Mouse CD2AP were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86727-1-PBS targets CD2AP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0622 Product name: Recombinant mouse Cd2ap protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-637 aa of BC157901 Sequence: MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEPKDDNLPIKRERQGNVASLVQRISTYGLPAGGIQPHPQTKAIKKKTKKRQCKVLFDYSPQNEDELELIVGDVIDVIEEVEEGWWSGTLNNKLGLFPSNFVKELESTEDGETHNAQEESEVPLTGPTSPLPSPGNGSEPAPGSVAQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKTEKPLILQPLGSRTQNVEVTKPDVDGKIKAKEYCRTLFPYTGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQISELDKDFPKPKKPPPPAKGPAPKPDLSAAEKKAFPLKAEEKDEKSLLEQKPSKPAAPQVPPKKPTAPTKASNLLRSPGAVYPKRPEKPVPPPPPAAKINGEVSIISSKIDTEPVSKPKLDPEQLPVRPKSVDLDAFVARNSKETDDVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTQSPEKTLKLPKEDDSGNLKPLEFKKDASYSSKPSLSTPSSASKVNTAAFLTPLELKAKAEADDGKKNSVDELRAQIIELLCIVDALKKDHGKELEKLRKELEEEKAMRSNLEVEIAKLKKAVLLS Predict reactive species
Full Name	CD2-associated protein
Calculated Molecular Weight	637 aa, 70 kDa
Observed Molecular Weight	74 kDa
GenBank Accession Number	BC157901
Gene Symbol	Cd2ap
Gene ID (NCBI)	12488
RRID	AB_3745083
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9JLQ0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).