APC Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse and More (2)

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

145-2C11

Cat no : APC-65060

Synonyms

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 1 publications below

Product Information

APC-65060 targets CD3ε in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivityhuman, mouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37
Full Name CD3 antigen, epsilon polypeptide
GenBank accession numberBC098236
Gene symbol Cd3e
Gene ID (NCBI) 12501
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and a stabilizer.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for APC CD3ε antibody APC-65060Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Eur J Med Res

Dickkopf-related protein 3 alters aerobic glycolysis in pancreatic cancer BxPC-3 cells, promoting CD4+ T-cell activation and function.

Authors - Qingqu Guo