APC Anti-Mouse CD3ε (145-2C11)
CD3ε Monoclonal Antibody for FC
Host / Isotype
Hamster / IgG
Reactivity
mouse and More (1)
Applications
FC
Conjugate
APC Fluorescent Dye
CloneNo.
145-2C11
Cat no : APC-65060
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | mouse splenocytes |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 2 publications below |
Product Information
APC-65060 targets CD3ε in FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Hamster / IgG |
Class | Monoclonal |
Type | Antibody |
Immunogen | H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37 |
Full Name | CD3 antigen, epsilon polypeptide |
GenBank Accession Number | BC098236 |
Gene Symbol | CD3ε |
Gene ID (NCBI) | 12501 |
RRID | AB_2882962 |
Conjugate | APC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
Product Specific Protocols | |
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FC protocol for APC CD3ε antibody APC-65060 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Appl Mater Interfaces Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer | ||
Eur J Med Res Dickkopf-related protein 3 alters aerobic glycolysis in pancreatic cancer BxPC-3 cells, promoting CD4+ T-cell activation and function. |