CoraLite® Plus 488 Anti-Mouse CD3ε (500-A2)

CD3ε Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Hamster / IgG

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

500-A2

Cat no : CL488-65061

Synonyms

Cd3e, T3e, CD3epsilon, CD3, AI504783

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL488-65061

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD3ε (CL488-65061, Clone: 500-A2) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL488-65061

  1X10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD3ε (CL488-65061, Clone:500-A2) (green) or 0.25 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65061 targets CD3ε in IF/ICC, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Immune complexes isolated from C6VL-BS thymoma cells
• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• RRID: AB_2883156
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).