CoraLite® Plus 488 Anti-Mouse CD3ε (500-A2)

CD3ε Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65061
Clone No.500-A2

Host / Isotype

Hamster / IgG

Reactivity

mouse

Applications

IF/ICC, FC

Cd3e, T3e, CD3epsilon, CD3, AI504783

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-65061 targets CD3ε in IF/ICC, FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Immune complexes isolated from C6VL-BS thymoma cells Predict reactive species
Full Name CD3 antigen, epsilon polypeptide
GenBank Accession NumberBC098236
Gene Symbol CD3ε
Gene ID (NCBI) 12501
RRIDAB_2883156
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP22646
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD3ε antibody CL488-65061Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Acta Pharm Sin B

Free fatty acid receptor-4 regulates T-cell-mediated allogeneic reaction through activating an aryl hydrocarbon receptor pathway

Authors - Maxwell Duah
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