CoraLite® Plus 647 Anti-Mouse CD3ε (500-A2)

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  IF Staining of mouse splenocytes using CL647-65061

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD3ε (CL647-65061, Clone: 500-A2) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65061

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.3 ug CoraLite® Plus 647-conjugated Anti-Mouse CD3ε (CL647-65061, Clone: 500-A2) (red) or 0.3 ug isotype control antibody (blue). Cells were not fixed.

CD3ε Monoclonal Antibody for IF/ICC, FC

Cat No. CL647-65061

Clone No.500-A2

Publications(1)

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

IF/ICC, FC

Synonyms

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e

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Tested Applications

• Positive IF/ICC detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65061 targets CD3ε in IF/ICC, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Immune complexes isolated from C6VL-BS thymoma cells
• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• RRID: AB_2883661
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P22646
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
		Cancer Cell; Overcoming tyrosine kinase inhibitor resistance in lung cancer brain metastasis with CTLA4 blockade; Authors - Minjie Fu