FITC Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

17A2

Cat no : FITC-65077

Synonyms



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 5 publications below

Product Information

FITC-65077 targets CD3 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen γδTCR-positive T-T hybridoma D1
Full Name CD3 antigen, epsilon polypeptide
GenBank accession numberBC098236
Gene symbol Cd3e
Gene ID (NCBI) 12501
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for FITC CD3 antibody FITC-65077Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Phytomedicine

Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.

Authors - Li Wu
mouseFC

Vet Microbiol

An influenza virus vector candidate vaccine stably expressing SARS-CoV-2 receptor-binding domain produces high and long-lasting neutralizing antibodies in mice.

Authors - Yongzhen Zhao
mouseFC

Malar J

Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173.

Authors - Huajing Wang
FC

Liver Int

Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury

Authors - Yuan Li
mouseFC

Bioact Mater

Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment

Authors - Dongdong Zheng