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Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody, PBS Only (98253-1-PBS)

Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98253-1-PBS-1MG

CD30 Ligand/TNFSF8 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98253-1-PBS

Clone No. 241831D9

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

CD30 L, TNFSF 8, TNFSF8, 241831D9, CD30L

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)
1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)
1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98253-1-RR
Biolayer interferometry (BLl) kinetic assays of 98253-1-RR against Human CD30 Ligand/TNFSF8 were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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Product Information

98253-1-PBS targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2118 Product name: Recombinant Human CD30 Ligand/TNFSF8 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 63-234 aa of BC093630 Sequence: QRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD Predict reactive species
Full Name	tumor necrosis factor (ligand) superfamily, member 8
Calculated Molecular Weight	234 aa, 26 kDa
GenBank Accession Number	BC093630
Gene Symbol	TNFSF8
Gene ID (NCBI)	944
RRID	AB_3745920
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P32971
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).