Mouse CD30/TNFRSF8 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of EL-4 using 84420-7-RR (same clone as 84420-7-PBS)

  EL-4 cells were subjected to SDS PAGE followed by western blot with 84420-7-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84420-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84420-7-RR (same clone as 84420-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84420-7-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84420-7-PBS in a different storage buffer formulation.

CD30/TNFRSF8 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84420-7-PBS

Clone No.241710B9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CD30, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8, Tumor necrosis factor receptor superfamily member 8

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Product Information

84420-7-PBS targets CD30/TNFRSF8 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1307

  Product name: Recombinant Mouse CD30/TNFRSF8 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 19-281 aa of NM_009401.2

  Sequence: FPTDRPLKTTCAGDLSHYPGEAARNCCYQCPSGLSPTQPCPRGPAHCRKQCAPDYYVNEDGKCTACVTCLPGLVEKAPCSGNSPRICECQPGMHCCTPAVNSCARCKLHCSGEEVVKSPGTAKKDTICELPSSGSGPNCSNPGDRKTLTSHATPQAMPTLESPANDSARSLLPMRVTNLVQEDATELVKVPESSSSKAREPSPDPGNAEKNMTLELPSPGTLPDISTSENSKEPASTASTLSLVVDAWTSSRMQPTSPLSTGT

• Full Name: tumor necrosis factor receptor superfamily, member 8
• Calculated Molecular Weight: 53kDa
• Observed Molecular Weight: 105-120 kDa
• GenBank Accession Number: NM_009401.2
• Gene Symbol: Tnfrsf8
• Gene ID (NCBI): 21941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q60846
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).