CD30/TNFRSF8 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• CD30/TNFRSF8 Recombinant monoclonal antibody (87467-3-RR)

CD30/TNFRSF8 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87467-3-RR

Clone No.252212B8

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

CD30, CD30L receptor, Lymphocyte activation antigen CD30, TNFRSF8, Tumor necrosis factor receptor superfamily member 8

• 

  WB analysis of PC-12 using 87467-3-RR

  PC-12 cells were subjected to SDS PAGE followed by western blot with 87467-3-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 87467-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87467-3-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-12 using 87467-3-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD30/TNFRSF8 antibody (87467-3-RR, Clone: 252212B8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87467-3-RR

  Biolayer interferometry (BLl) kinetic assays of 87467-3-RR against Rat CD30/TNFRSF8 were performed. The affinity constant is 6.23 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: PC-12 cells, rat spleen tissue, rat thymus tissue, rat lung tissue, mouse spleen tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87467-3-RR targets CD30/TNFRSF8 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6226

  Product name: Recombinant Rat CD30/TNFRSF8 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 19-282 aa of /

  Sequence: FPKDRPLDTTCTGDLSYYPGEAARNCCYQCPSGLSPTQPCPQGPATARSSVILTTTNEDRKCTACVTCLPGLVEKAPCSGNSPRICECQPGMYCSTPAVNSCARCSEKTVVKFPDTAEKNTICDLPSPGSGPNGSNPDDCKTLTSHTTPQAIPTLESPANDSVRSLLPKQVTDFVNEGATKLVKVPESSSSKASMPSPDPGNAEMNMTLKLPPPGTVPDISTSENSMEPASTASTLSLLVDARTSSRMQPTSPLSTGTPFLDPG

• Full Name: tumor necrosis factor receptor superfamily, member 8
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: /
• Gene Symbol: Tnfrsf8
• Gene ID (NCBI): 25069
• RRID: AB_3745565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P97525
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612).