Anti-Mouse CD30/TNFRSF8 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98209-1-RR

  1x10^6 anti-CD3/CD28-treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30/TNFRSF8 Rabbit RecAb (98209-1-RR, Clone: 241710B4) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30/TNFRSF8 Rabbit Recombinant Antibody (98209-1-RR, Clone: 241710B4) and PE-Conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98209-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98209-1-RR against Mouse CD30/TNFRSF8 were performed. The affinity constant is 1.64 nM.

CD30/TNFRSF8 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98209-1-RR

Clone No.241710B4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Tumor necrosis factor receptor superfamily member 8, Tnfrsf8, Lymphocyte activation antigen CD30, CD30L receptor, CD30

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98209-1-RR targets CD30/TNFRSF8 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: tumor necrosis factor receptor superfamily, member 8
• Calculated Molecular Weight: 53kDa
• GenBank Accession Number: NM_009401.2
• Gene Symbol: Tnfrsf8
• Gene ID (NCBI): 21941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q60846
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).