CD300a Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84674-4-RR (same clone as 84674-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84674-4-RR (CD300A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84674-4-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 84674-4-RR (same clone as 84674-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD300A antibody (84674-4-RR, Clone: 242131H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84674-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84674-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84674-4-RR against Human CD300A were performed. The affinity constant is below 1 pM.

CD300a Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84674-4-PBS

Clone No.242131H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CMRF 35 H9, CLM-8, CLM 8, CD300a molecule, CD300 antigen-like family member A

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Product Information

84674-4-PBS targets CD300a in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CD300a molecule
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 40-70 kDa
• GenBank Accession Number: NM_007261.4
• Gene Symbol: CD300a
• Gene ID (NCBI): 11314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UGN4-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human CD300a is a transmembrane protein, with an immunoglobulin (Ig)V-like extracellular domain and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs), providing the receptor with an inhibitory capacity. The relevance of the CD300a molecule in several pathological conditions has been highlighted by multiple studies. (PMID: 37762055)